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br Materials and methods BAECs CAB Toyobo
2020-01-29
Materials and methods BAECs (CAB30405, Toyobo Life Science, Osaka, Japan) were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% Fetal Bovine Serum (04-001-1A-US, Biological Industries, Kibbutz Beit Haemek, Israel) and 1% antibiotic-antifungal agent containing 10,000-μg/mL penici
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The endothelin receptor type B
2020-01-29
The endothelin receptor type B (EDNRB) belongs to the family of G protein-coupled receptors, which functions as a vital regulatory factor in signal transduction in cells, locating on human chromosome 13q22.3 (Ayala-Valdovinos et al., 2016; Bregar et al., 2018; Morimoto et al., 2018; Widowati et al.,
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In mice our EBI EGFP reporter showed
2020-01-29
In mice, our EBI2EGFP reporter showed that EBI2 is expressed consistently in naive T helper cells. Already single positive CD4+ thymocytes express EBI2 (not shown). In effector and regulatory T cells in naive mice, the percentage of EBI2 expression was lower (∼40%). Like others (Liu et al., 2011), w
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br Acknowledgements br Introduction Lung cancer treatment st
2020-01-29
Acknowledgements Introduction Lung cancer treatment strategies, especially molecular targeted therapy, have progressed rapidly. However, most established treatments are for adenocarcinoma of the lung, not for squamous cell carcinoma (SQCC). SQCC constitutes approximately 30% of lung cancers, w
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Having generated synthetic cytokines and synthetic
2020-01-29
Having generated synthetic cytokines and synthetic cytokine receptors, the logical next step has been to design fully synthetic cytokine/cytokine receptor systems, modifying either cytokine/cytokine receptor binding interfaces or cytokine-unrelated combinations of substances and their binding domain
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Genomic DNA Isolation Kit One specific brain region that may
2020-01-29
One specific Genomic DNA Isolation Kit region that may be involved in the reduction of anxiety after anti-SVG-30 treatment is the lateral septum which contains a high density of CRF2 receptors [5], [44]. The lateral septum often exhibits increased immediate early gene expression in response to stre
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In this study we observed alterations in mRNA
2020-01-29
In this study, we observed alterations in mRNA levels of CRF receptors in the PFCx and Hip during the development of sensitization. In particular, in the Hip both CRF receptors showed significantly increased GFP Quantitation Kit australia when the sensitization condition was established. Similarly,
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OxLDL down regulates eNOS and up regulates iNOS
2020-01-29
OxLDL down-regulates eNOS and up-regulates iNOS, thereby augmenting the formation of NO and protein S-nitrosylation in human endothelial GKT137831 [26]. Importantly, iNOS-mediated S-nitrosylation plays an increasingly significant role in cardiovascular diseases [34]. For example, iNOS-mediated IRE1
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br Materials and methods br Discussion To date
2020-01-29
Materials and methods Discussion To date, only a fraction of studies pertaining to analysis of avian semen have focused on the study of DNA, with the vast majority being associated with the effects of cryopreservation on sperm DNA integrity (Kotłowska et al., 2007, Madeddu et al., 2009, Partyk
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br Results and discussion A systematic search for
2020-01-29
Results and discussion A systematic search for genes encoding H+-PPases was carried out by PCR using genomic DNA from a wide range of protozoa as templates and degenerate oligonucleotides, designed from amino hpdp domains conserved in H+-PPases of higher plants and the proteobacterium R. rubrum,
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We have performed our MSP
2020-01-29
We have performed our MSP analyses as real-time PCR using SybrGreen (Fig. 2). The results of the beta-actin PCR vary in each sample group because the amount of DNA for bisulfite treatment was adjusted to a constant volume of serum (500μl) rather than to equal amounts of DNA. Because we expected lowe
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Here we examine the associations between serum pro
2020-01-29
Here, we examine the associations between serum pro and anti-inflammatory cytokines and cognitive functioning in early-stage bipolar I disorder (BDI) patients. As per previous studies, ‘early-stage’ was defined as being within three years of the first manic episode (Kauer-Sant\'Anna et al., 2009). W
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br Materials and methods br Acknowledgements We thank Drs Na
2020-01-25
Materials and methods Acknowledgements We thank Drs. Nathan Sherer (University of Wisconsin-Madison) and Bryan Cullen (Duke University) for generous reagent gifts. The following reagent was obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: HIV-2ROD phage from Dr. Ron
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Due to the results of the experiment
2020-01-25
Due to the results of the experiment we decided to deploy our cloud service infrastructure with two platform-independent servers instead of one or three. This decision was based on two factors: (a) the use of more than one server is useful to avoid a bottleneck in the system, and (b) using more than
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Apparently no study has been undertaken
2020-01-25
Apparently, no study has been undertaken on alterations in cholinesterase activity and its correlation with neurological disorders in clinical case of trypanosomosis in buffaloes. Therefore, the present study was undertaken to evaluate the alterations in cholinesterase activity in clinical cases of
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